KMID : 0903519880310040346
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology 1988 Volume.31 No. 4 p.346 ~ p.350
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Genetic Breeding of Korean Soy Sauce - Fermenting Bacillus by UV Mutation
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Abstract
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A mutant for Korean soy sauce which wilt improve the productivity of amylase and protease was obtained through the second mutation of the original strain using UV radiation. The original strain was the NTG treated mutant of the Bacillus sp. producing peculiar flavour which had been isolated from the korean soy sauce. The mutant could improve the productivity of amylase by 58% and that of protease by 41%. The enzyme produced in this way were similar in enzymatic properties such as optimal reaction pH and temperature. The reaction was not deterred by highly densed salt solution of 5 M and the enzyme productivity was not influenced in the concentration of up to 2 M.
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